Determine if we can see delayed fluorscence from MTTI 135, as expect (see other posts on MTTI 135). First, lets verify we can see it via single photon excitation using the Ocean Optics PX2 flash lamp and fluorescence spectrometer.

I've ordered additional UG 5 and Blue glass filters from thor labs to help filter the excitation light. They shipped on 6/8/2011.

Do a lit search to find recent reviews of metabolic control, energy metabolism in the cochlea, relation of nadh fluorescence to metabolic capacity at the cellular level.

Based on Sam's measurements of MTTI 128, 129, 116, 137 and 138, it is clear we need an additional round of diffusion distance vs. time measurements to complete the evaluation of these probes.

MTTI 151is a new dye that Brian has created. The goal is simple. Incubate in living cells or in stock solutions and try to image the dye using two-photon excitation.

This requires the cell line to be brought up again.

Find the average cell intensity and cell size (for each cell type in each image) for each cell analyzed. This data should be in the Full Summary spreadsheet. Add this data to the mastersheet

Sam took images of MTTI 128, 129, 116, 137 and 138 during the summer of 2010. We need to analyze the images that she obtained to determine the time scaling exponent, transport coefficient, and diffusion constants for each of these dyes.