Atomic Force Microscopy is a technique by which a long cantilever with an atomically sharp tip is systematically moved across the surface of a specimen. Any height changes in the tip are recorded as a function of position, resulting in a topographical reconstruction of the surface. Using a custom-made, temperature-controlled AFM for in-liquid imaging, we are able to map out a full 3-D model of gingival fibroblast cells in liquid and dry environments to observe cellular attachment.
